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Mouse anti dsDNA IgG1 specific ELISA Kit
cat#5120-1
INTENDED USE
The Mouse anti-dsDNA IgG1 ELISA Kit is an immunoassay suitable
for quantifying or titering IgG1 antibody activity specific
for double-stranded DNA in serum or plasma. Other biologicalfluids, including tissue culture medium, may be validated for use.
For research use only (RUO), not for diagnosis, cure or
prevention of the disease.
GENERAL INFORMATION
Antibodies reactive with autologous nuclear components, such as
DNA and histones, can represent an autoimmune basis for
pathological conditions such as systemic lupus erythematosis
(SLE) in humans, and in mice homozygous for the
lymphoproliferation spontaneous mutation (Faslpr), a systemic
autoimmunity with massive lymphadenopathy associated with
proliferation of aberrant T cells, arthritis and immune complex
glomerulonephritis. These conditions include elevated levels of
anti-dsDNA and other anti-nuclear antibodies (ANA) which often
increase as the animal ages. Also, the expanded use in the drug
industry of biological modifiers has been associated with
production of autoantibodies, of which mice, and possibly also
other hosts such as humans and monkeys, are susceptible. A
prototype disease in mice is lupus caused by the drug
minocycline, with elevated anti-dsDNA among other
autoantibodies and pathological conditions.
Recent investigations have focused on the role of innate immune
mechanisms, including Toll-like receptors (TLRs) and TH2
immunity, responding to the damage-associated molecular
patterns of dying cells, as underlying cause of the anti-dsDNA
type autoimmunity; these may be induced by drugs, including
vaccines and adjuvants, with aging, or with other conditions.