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Mice were immunized with antigen grade Human IgE
and the spleen and lymph node lymphocytes were
fused to form hybridomas. Specificity of antibody has
been tested using ELISA. The product reacts with
Human IgE with no significant reactivity with Human
IgG, IgM, or IGA. Antibody was purified from tissue
culture fluid using Protein A affinity column. Purified
anti-Human IgE antibody is supplied as unlabeled or
conjugated to Biotin, FITC, or HRP.
Affinity constant: 3X1010
Subclass: IgG2b
Form and Storage
The antibody is supplied in PBS, pH 7.4 with 0.1%
sodium azide, either lyophilized (0.1 mg) or liquid
form. Reconstitute powder in PBS to prepare stock
solution. Store at 2-8oC in suitable aliquots. Stability is
~6-12 months. Do not freeze and thaw.