Probetex，Inc 肾炎模型诱导试剂一级代理

Probetex，Inc公司成立于1997年，是一家为生物科研研究提供服务的CRO 公司。公司专注于6个研究领域：1）成人和胚胎肾细胞系的细胞库2）生产和销售绵羊肾毒性抗体以诱导抗肾小球基底膜（抗GBM）肾小球肾炎，膜性（抗Fx1A）肾病和系膜增生性（抗Thy1）肾小球肾炎。3）肾病模型研究的组织产品4）组织病理学服务，提供常规组织学，免疫组织化学，电子显微镜和图像分析; 5）提供以上肾病以及20多种急性、慢性实验性肾病、心脑血管和肺病的其他模型的构建服务。6）其他研究领域，比如：再生医学、血管炎新模型以及其他罕见疾病模型的构建和评价服务。

特色产品：

绵羊抗大鼠肾小球基底膜血清（PTX-001AGBM）

Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum

--抗GBM型肾炎模型诱导

应用说明：(实验前请仔细阅读实验流程)

每瓶血清含有足够的抗体用于诱导至少25只大鼠*（175-200 g体重）被动抗GBM肾炎模型。

免疫抗GMB血清：麻醉大鼠，尾静脉注射抗GMB血清超过15秒，疾病的产生依赖于免疫剂量，依照免疫剂量*打入体内很关键（推荐用量：0.4-0.5ml/100g体重）。由于不同批次抗血清差异，大鼠来源，以及实验要求的不同，我们建议进行剂量-反应研究，具体的免疫剂量和疾病的严重程度，需要根据每个不同批次血清和产生疾病的严重程度，选择合适的免疫剂量。

肾病研究：

1）异源性疾病：免疫抗GBM (anti GBM)3-5天后，免疫荧光检测显示绵羊IgG在肾小球基底膜内呈线状沉积。大鼠c3也呈线性分布，24小时后出现蛋白尿。

2）自身疾病：免疫抗GBM抗体8-10天后，检测大鼠蛋白尿增加，同时大鼠肾小球IgG(自身抗体) 呈线状沉积，自体疾病变得明显。（自体）免疫组化呈线性模式的IgG（图1）。到3周时，蛋白尿有望达到200毫克/24小时，肾小球病变（图2）明显，随着时间的推移，严重程度增加，导致肾小球硬化。

图1.免疫荧光检测显示免疫抗GBM3周后肾小球毛细血管壁大鼠IgG免疫沉积的。

图2.免疫组化染色显示免疫抗GBM 3周后新月体形成、系膜细胞增生、毛细血管变形。

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